Evaluation of Using Nanomaterial in Tissue Culture Media and Biological activity

The field of nanotechnology is one of the most active areas of research in modern materials science. New applications of nanoparticles and nanomaterials are emerging rapidly. Nanocrystalline silver particles have found tremendous applications in the field of high sensitivity biomolecular detection and diagnostics, antimicrobials and therapeutics, Catalysis and micro-electronics. The use of nano-sized silver particles as antimicrobial agents has become more common as technological advances make their production more economical. TiO2 is the most commonly used semiconductor photocatalyst. Among the different nanomaterials, it is the most studied. The concentration of TiO2 usually required to kill bacteria varies and depending on the size of the particles and the intensity and wavelength of the light used. Plant tissue culture techniques involve the growing and multiplication of totipotent cells, tissues and organs of plants on defined solid or liquid media comprising nutrients under an aseptic and controlled environment. Plant tissue culture has an important role in the production of agricultural or ornamental plants and in the manipulation of plants for improved agronomic performance. This research was planned to evaluate the potential of nano silver and TiO2 to remove bacterial contaminants that exist in plant tissue culture media. The modified MS medium evaluated after four weeks. The results showed that nano silver and TiO2 had a good potential for removing the bacterial contaminants in plant tissue